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OvaNext (25 genes) logo

Is OvaNext the right test for you?

 

Female cancers often appear quietly, masking themselves with non-specific symptoms, making them hard to detect without proper testing. OvaNext looks at your risk of developing hereditary breast, ovarian, or uterine cancer.

Having right test at the right time can provide you with critical information so that you can get the personalised treatment and screening options you need.

To get this test you will first need a consultation with your clinician, then you can either:

  • Buy it directly from this website (this cost may be covered by your medical insurance).
  • Access it through your treating hospital/private healthcare provider.

This test is only available to those aged 18 years or above.

 

Why?Close tab

Helping you make decisions

 

OvaNext includes 25 genes that are linked to an increased lifetime risk for one or more cancers such as breast, ovarian, uterine, and/or others.

Understanding your risk can help you and your clinician could decide at what age to have scans – and how frequently to have them in the future.

It can help you make vital decisions about preventative or ‘prophylactic’ treatment – perhaps a full or partial mastectomy (removing one or both breasts) or oophorectomy (removing the ovaries and Fallopian tubes) before a cancer occurs.

 

For your family

 

It’s also important to share the test analysis with your family: because, there could be implications for them too. For example, if a man or woman carries a mutation in BRCA1 (one of the genes tested), each of their parents (mother and father), siblings (brothers and sisters) and children (sons and daughters) have a 50% chance of carrying the same mutation. Knowing if this gene mutation is in the family means everyone can ensure they receive regular monitoring and screening.

 

How?Close tab

For your family

It’s also important to share the test analysis with your family: because, there could be implications for them too. For example, if a man or woman carries a mutation in BRCA1 (one of the genes tested), each of their parents (mother and father), siblings (brothers and sisters) and children (sons and daughters) have a 50% chance of carrying the same mutation. Knowing if this gene mutation is in the family means everyone can ensure they receive regular monitoring and screening.

 

ProcessClose tab

Buying the test

Step 1:  There are two options:

  • Via your treating hospital/private healthcare provider (we have special arrangements with many healthcare providers to enable you to access this test, click here to see the list)
  • By clicking the ‘Buy direct now’ button above (the cost may be covered by your medical insurance – check your policy details)

Step 2: Collection of sample

We will liaise directly with your clinician and organise the collection of your sample and delivery to the laboratory.

Step 3: Analysis and reporting

Your sample will be analysed and a report will be generated, usually within four to five weeks of receipt of your sample in the laboratory.

Step 4: Results

Your clinician will receive the test report giving a clear indication of whether you are at risk of breast, ovarian or uterine cancer, and what steps you – and possibly other members of your family – need to take next.

 

CliniciansClose tab

Is OvaNext the right test for your patient?

This is a comprehensive test for gynaecological cancers to increase the chance of identifying and managing your patient’s hereditary cancer risks.

When to consider testing

  • Ovarian cancer at any age
  • Uterine cancer diagnosed <50 years of age or with abnormal MSI/IHC
  • Multiple primary cancers in one person (e.g. uterine and breast or thyroid cancer)
  • Multiple close family members with ovarian or uterine and other cancers (on the same side of the family)
  • Cancer histories that are suspicious for both hereditary breast ovarian cancer and Lynch syndrome


Mutation Detection Rates*

 

 

 

 

 

 

 

*Excludes MUTYH carriers, and CHEK2 p.l157T

Test Description

OvaNext analyses 25 genes. 24 genes (excluding EPCAM) are evaluated by next generation sequencing (NGS) or Sanger sequencing of all coding domains, and well into the flanking 5’ and 3’ ends of all the introns and untranslated regions. In addition, sequencing of the promoter region is performed for the following genes: PTEN (c.-1300 to c.-745), MLH1 (c.-337 to c.-194), and MSH2 (c.-318 to c.-65). The inversion of coding exons 1-7 of the MSH2 gene and the BRCA2 Portuguese founder mutation, c.156_157insAlu (also known as 384insAlu) are detected by NGS and confirmed by PCR and agarose gel electrophoresis.

Clinically significant intronic findings beyond 5 base pairs are always reported. Intronic variants of unknown or unlikely clinical significance are not reported beyond 5 base pairs from the splice junction. Additional Sanger sequencing is performed for any regions missing or with insufficient read depth coverage for reliable heterozygous variant detection. Reportable small insertions and deletions, potentially homozygous variants, variants in regions complicated by pseudogene interference, and single nucleotide variant calls not satisfying 100x depth of coverage and 40% het ratio thresholds are verified by Sanger sequencing.1

Gross deletion/duplication analysis is performed for the covered exons and untranslated regions of all 25 genes using read-depth from NGS data with confirmatory multiplex ligation-dependent probe amplification (MLPA) and/or targeted chromosomal microarray. If a deletion is detected in exons 13, 14, or 15 of PMS2, double stranded sequencing of the appropriate exon(s) of the pseudogene, PMS2CL, will be performed to determine if the deletion is located in the PMS2 gene or pseudogene.

 

1. Mu W, et al. Sanger confirmation is required to achieve optimal sensitivity and specificity in next-generation sequencing panel testing. J Mol Diagn. 2016. 18(6):923-932.

This test is provided by:

Ambry Genetics

Frequently asked questions

Do I need a doctor to authorise this test?
Yes, your doctor will need to provide the sample required for this test
Will my health insurance cover the cost of this test?
Many health insurance plans cover the cost of genetic testing – check with your provider to find out if it is in your policy.
How soon do I get my results?
Usually within four to five weeks of receipt of your sample in the laboratory.
How accurate is the test?
Ambry follows advanced laboratory procedures and ensures quality control to deliver accurate results you can trust.